1FSK
COMPLEX FORMATION BETWEEN A FAB FRAGMENT OF A MONOCLONAL IGG ANTIBODY AND THE MAJOR ALLERGEN FROM BIRCH POLLEN BET V 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 91.653, 99.145, 108.905 |
| Unit cell angles | 105.70, 98.32, 97.62 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.28500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.047 | 0.370 |
| Total number of observations | 181008 * | |
| Number of reflections | 80241 * | 6763 * |
| <I/σ(I)> | 19.85 | |
| Completeness [%] | 95.5 | 83.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4 | 277 * | 12% peg 6000, 0.1M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 12 (%) | |
| 3 | 1 | drop | sodium citrate | 0.1 (M) | |
| 4 | 1 | drop | sodium azide | 0.01 (%) | |
| 5 | 1 | reservoir | PEG6000 | 12 (%) | |
| 6 | 1 | reservoir | sodium azide | 0.01 (%) |
