1FSE
CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS REGULATORY PROTEIN GERE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02-15 |
Detector | ADSC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.019, 61.749, 71.743 |
Unit cell angles | 90.00, 97.08, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.050 |
Rwork | 0.214 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.560 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.068 | 0.390 |
Total number of observations | 91541 * | |
Number of reflections | 29311 * | |
<I/σ(I)> | 11.53 | 4.07 |
Completeness [%] | 99.0 | 95.5 |
Redundancy | 3.11 | 2.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 * | Peg4000, sodium acetate, lithium or ammonium sulfate, pH 5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 30 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | lithium sulfate | 0.2 (M) | or ammonium sulfate |