1FSC
Crystal Structure of Fasciculin 2 from Green Mamba Snake Venom: Evidence for Unusual Loop Flexibility
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1992 |
Detector | XENTRONICS |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 48.930, 48.930, 82.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.188 |
Rwork | 0.188 |
RMSD bond length | 0.016 |
RMSD bond angle | 26.930 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 * |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.088 * |
Number of reflections | 6363 |
Completeness [%] | 90.0 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | ammonium acetate | 0.05 (M) | |
3 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
4 | 1 | reservoir | beta-octylglucoside | 5 (mM) | |
5 | 1 | reservoir | MES | 0.1 (M) |