1FSC

Crystal Structure of Fasciculin 2 from Green Mamba Snake Venom: Evidence for Unusual Loop Flexibility

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992
Detector	XENTRONICS
Spacegroup name	P 41 21 2
Unit cell lengths	48.930, 48.930, 82.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.188
Rwork	0.188
RMSD bond length	0.016
RMSD bond angle	26.930 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.088 *
Number of reflections	6363
Completeness [%]	90.0
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *
1	Vapor diffusion, hanging drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium acetate	0.05 (M)
3	1	reservoir	ammonium sulfate	35 (%sat)
4	1	reservoir	beta-octylglucoside	5 (mM)
5	1	reservoir	MES	0.1 (M)