1FSA

THE T-STATE STRUCTURE OF LYS 42 TO ALA MUTANT OF THE PIG KIDNEY FRUCTOSE 1,6-BISPHOSPHATASE EXPRESSED IN E. COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-02
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 2
Unit cell lengths	61.050, 166.720, 79.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.184
Rwork	0.184
R-free	0.23200
RMSD bond length	0.012
RMSD bond angle	24.870 *
Data reduction software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	80.000
High resolution limit [Å]	2.200
Rmerge	0.090
Total number of observations	66515 *
Number of reflections	27660
<I/σ(I)>	3.5
Completeness [%]	81.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	EDTA	0.025 (mM)
4	1	drop	PEG3500	7.5 (%)
5	1	drop		0.09 (M)
6	1	reservoir	Tris-HCl	50 (mM)
7	1	reservoir	PEG3500	15 (%)