1FS5
A DISCOVERY OF THREE ALTERNATE CONFORMATIONS IN THE ACTIVE SITE OF GLUCOSAMINE-6-PHOSPHATE ISOMERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-09 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 124.228, 124.228, 222.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.730 |
R-factor | 0.212 * |
Rwork | 0.212 |
R-free | 0.23100 * |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.083 * | 0.284 * |
Number of reflections | 65814 * | |
<I/σ(I)> | 8.1 | |
Completeness [%] | 95.6 * | 86.3 * |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 291 | HEPES, sodium potassium tatrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | sodium tartrate | 0.75 (M) | |
3 | 1 | reservoir | HEPES | 200 (mM) | pH7.0 |
4 | 1 | reservoir | GlcNAc6P | 0.050 (mM) |