1FS3

CRYSTAL STRUCTURE OF WILD-TYPE BOVINE PANCREATIC RIBONUCLEASE A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-31
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 32 2 1
Unit cell lengths	64.046, 64.046, 63.355
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.400
R-factor	0.217
Rwork	0.217
R-free	0.25500
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000	1.400
High resolution limit [Å]	1.400	1.350
Rmerge	0.050	0.190
Number of reflections	33471
<I/σ(I)>	14.3
Completeness [%]	98.8	97.4
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K