1FRW
STRUCTURE OF E. COLI MOBA WITH BOUND GTP AND MANGANESE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2000-07-19 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.387, 124.387, 67.399 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.1856 |
Rwork | 0.183 |
R-free | 0.22600 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.084 | 0.441 |
Number of reflections | 26350 | |
<I/σ(I)> | 14 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.8 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 295 | sodium acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 12 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 2.5-3 (M) | |
3 | 1 | reservoir | cacodylic acid | 0.1 (M) | pH6.5 |