1FRV
CRYSTAL STRUCTURE OF THE OXIDIZED FORM OF NI-FE HYDROGENASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1992-09-23 |
Spacegroup name | P 1 |
Unit cell lengths | 63.600, 93.900, 69.200 |
Unit cell angles | 89.80, 102.90, 90.80 |
Refinement procedure
Resolution | 8.000 - 2.850 |
R-factor | 0.178 * |
Structure solution method | MIR WITH AVERAGING |
RMSD bond length | 0.012 |
RMSD bond angle | 0.032 |
Data reduction software | MOSFLM |
Data scaling software | BIOMOL ((SCALKB/KBAPLY)) |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.500 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.086 | 0.207 |
Total number of observations | 116003 * | |
Number of reflections | 31053 | |
Completeness [%] | 85.5 | 47.6 |
Redundancy | 3.7 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.6 | pH 6.6 |