1FRH
AZOTOBACTER VINELANDII FERREDOXIN I: ALTERATION OF INDIVIDUAL SURFACE CHARGES AND THE [4FE-4S] CLUSTER REDUCTION POTENTIAL
Experimental procedure
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.250, 55.250, 95.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.720 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Total number of observations | 36832 * |
| Number of reflections | 8332 |
| Completeness [%] | 91.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 2 * | Stout, C.D., (1979) J. Biol. Chem., 254, 3598. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 0.5 (M) | |
| 3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.5 (M) |
