1FR9
STRUCTURE OF E. COLI MOBA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2000-07-01 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.118, 124.118, 66.983 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.650 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.24200 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.963 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.086 | 0.411 |
Number of reflections | 58834 | |
<I/σ(I)> | 9.4 | |
Completeness [%] | 97.8 | 100 |
Redundancy | 2.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 295 | sodium acetate, cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
1 | Vapor diffusion * | 6.5 | 295 | sodium acetate, cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 12 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 2.5-3 (M) | |
3 | 1 | reservoir | cacodylic acid | 0.1 (M) | pH6.5 |