1FR9
STRUCTURE OF E. COLI MOBA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-07-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 124.118, 124.118, 66.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.24200 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.963 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.086 | 0.411 |
| Number of reflections | 58834 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 97.8 | 100 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 295 | sodium acetate, cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
| 1 | Vapor diffusion * | 6.5 | 295 | sodium acetate, cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 12 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 2.5-3 (M) | |
| 3 | 1 | reservoir | cacodylic acid | 0.1 (M) | pH6.5 |
