1FR2

CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN WITH A MUTANT IMMUNITY PROTEIN IM9(E41A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.050, 51.640, 87.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.600
Rwork	0.205
R-free	0.23200
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.078	0.195
Number of reflections	26832
<I/σ(I)>	12.7
Completeness [%]	97.4	90.9
Redundancy	4.96	2.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	277	24% PEG 4000, 0.1M sodium acetate, , pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K