1FQI

RGS9 RGS DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-01
Detector	APS-1
Wavelength(s)	1.0283, 0.9795, 0.9793, 0.9351
Spacegroup name	C 1 2 1
Unit cell lengths	67.309, 71.836, 34.625
Unit cell angles	90.00, 96.13, 90.00

Refinement procedure

Resolution	50.000 - 1.940
R-factor	0.221 *
Rwork	0.223
R-free	0.24800 *
RMSD bond length	1.504
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	1.940	1.940
Rmerge	0.051	0.301
Number of reflections	23300
<I/σ(I)>	23.5
Completeness [%]	97.7	91.9
Redundancy	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	15.5% PEG8000, 50mM Tris pH8.0, 5% Ethylene Glycol, 75mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	15.5% PEG8000, 50mM Tris pH8.0, 5% Ethylene Glycol, 75mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	15.5% PEG8000, 50mM Tris pH8.0, 5% Ethylene Glycol, 75mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	15.5% PEG8000, 50mM Tris pH8.0, 5% Ethylene Glycol, 75mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG8000	15.5 (%)
3	1	reservoir	Tris	50 (mM)
4	1	reservoir	ethylene glycol	5 (%)
5	1	reservoir		75 (mM)