1FQD
CRYSTAL STRUCTURE OF MALTOTETRAITOL BOUND TO CLOSED-FORM MALTODEXTRIN BINDING PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.300, 69.400, 56.300 |
| Unit cell angles | 90.00, 112.40, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.191 * |
| Rwork | 0.191 |
| R-free | 0.24400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.143 |
| Total number of observations | 92330 * | |
| Completeness [%] | 99.3 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 6000, Mes, NaN3, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MBP | 3.5 (mg/ml) | |
| 2 | 1 | drop | maltotetraitol | 1 (mM) | |
| 3 | 1 | drop | PEG6000 | 16 (%(w/v)) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | drop | MES | 10 (mM) | |
| 6 | 1 | drop | protein | 12 (mg/ml) | |
| 7 | 1 | reservoir | PEG6000 | 22 (%) | |
| 8 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) | |
| 9 | 1 | reservoir | MES | 10 (mM) |
