1FQA
STRUCTURE OF MALTOTETRAITOL BOUND TO OPEN-FORM MALTODEXTRIN BINDING PROTEIN IN P2(1)CRYSTAL FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.900, 65.800, 57.800 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.176 * |
| Rwork | 0.176 |
| R-free | 0.21500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.126 |
| Total number of observations | 170555 * | |
| Number of reflections | 21890 | |
| Completeness [%] | 84.0 | 69.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | 273 | PEG 3350, NaN3, MES, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 273.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MBP | 3.5 (mg/ml) | |
| 2 | 1 | drop | maltotetraitol | 1 (mM) | |
| 3 | 1 | drop | PEG3350 | 20 (%) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | drop | MES | 10 (mM) | |
| 6 | 1 | drop | protein | 12 (mg/ml) | |
| 7 | 1 | reservoir | PEG3350 | 30 (%) | |
| 8 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) | |
| 9 | 1 | reservoir | MES | 10 (mM) |
