1FQ3
CRYSTAL STRUCTURE OF HUMAN GRANZYME B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.740, 114.310, 135.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Cathepsin G |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.199 | 0.199 |
| Number of reflections | 11275 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | PEG-8000, ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
