1FQ0
KDPG ALDOLASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-28 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.200, 77.790, 146.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.260 * |
| Rmerge | 0.057 * | 0.142 * |
| Total number of observations | 148386 * | |
| Number of reflections | 27601 * | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 94.0 * | 80 * |
| Redundancy | 5.1 * | 2.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 293.5 | Buchanan, L.Vl., (1999) Acta Crystallogr., D55, 1946. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | protein | 3 (mg/ml) | |
| 3 | 1 | drop | dithiothreitol | 4 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 20 (%) | |
| 5 | 1 | reservoir | citric acid | 0.075 (M) |
