1FQ0
KDPG ALDOLASE FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-05-28 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.200, 77.790, 146.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.25100 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.860 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.260 * |
Rmerge | 0.057 * | 0.142 * |
Total number of observations | 148386 * | |
Number of reflections | 27601 * | |
<I/σ(I)> | 17.2 | |
Completeness [%] | 94.0 * | 80 * |
Redundancy | 5.1 * | 2.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 * | 293.5 | Buchanan, L.Vl., (1999) Acta Crystallogr., D55, 1946. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | |
2 | 1 | drop | protein | 3 (mg/ml) | |
3 | 1 | drop | dithiothreitol | 4 (mM) | |
4 | 1 | reservoir | PEG6000 | 20 (%) | |
5 | 1 | reservoir | citric acid | 0.075 (M) |