1FPY
CRYSTAL STRUCTURE OF GLUTAMINE SYNTHETASE FROM SALMONELLA TYPHIMURIUM WITH INHIBITOR PHOSPHINOTHRICIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-04-24 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 230.600, 132.500, 195.900 |
Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.890 |
R-factor | 0.248 * |
Rwork | 0.248 |
R-free | 0.26300 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.950 |
High resolution limit [Å] | 2.890 | 2.900 |
Rmerge | 0.074 | 0.288 |
Number of reflections | 87421 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 70.0 | 58.6 |
Redundancy | 14.6 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | ADP, MPD, spermine, manganese chloride, imidazole buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | imidazole/HCl | 15 (mM) | |
2 | 1 | 1 | 3 (mM) | ||
3 | 1 | 1 | spermine tetrahydrochloride | 3 (mM) | |
4 | 1 | 1 | MPD | 10 (%) |