1FPL

FRUCTOSE-1,6-BISPHOSPHATASE (D-FRUCTOSE-1,6-BISPHOSPHATE 1-PHOSPHOHYDROLASE) COMPLEXED WITH AMP, 2,5-ANHYDRO-D-GLUCITOL-1,6-BISPHOSPHATE AND THALLIUM IONS (10 MM)

Experimental procedure

Collection date	1995-02-20
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	61.100, 166.900, 80.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.194
Rwork	0.194
RMSD bond length	0.011
RMSD bond angle	1.680
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.200
Rmerge	0.089
Total number of observations	94731 *
Number of reflections	32655
Completeness [%]	92.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10-15 (mg/ml)
2	1	2	Tris base	20 (mM)
3	1	2	PEG3350	11-12.5 (%)
4	1	2		6.0 (mM)
5	1	2	2-mercaptoethanol	1.0 (mM)
6	1	2	maleic acid	0.1 (mM)
7	1	2	AhG-1,6-P2	1 (mM)
8	1	2	AMP	1 (mM)