1FPK
FRUCTOSE-1,6-BISPHOSPHATASE (D-FRUCTOSE-1,6-BISPHOSPHATE 1-PHOSPHOHYDROLASE) COMPLEXED WITH THALLIUM IONS (10 MM)
Experimental procedure
| Collection date | 1994-12-17 |
| Detector | SIEMENS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 132.600, 132.600, 67.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.210 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.091 |
| Total number of observations | 33304 * |
| Number of reflections | 14091 |
| Completeness [%] | 74.0 |
| Redundancy | 2.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 10-15 (mg/ml) | |
| 2 | 1 | 2 | Tris base | 20 (mM) | |
| 3 | 1 | 2 | PEG3350 | 4 (%) |
