1FP8
STRUCTURE OF THE AMYLOMALTASE FROM THERMUS THERMOPHILUS HB8 IN SPACE GROUP P21212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1999-06-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 115.234, 93.670, 53.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.23200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (V1.17) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.460 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.076 | 0.370 |
| Number of reflections | 26405 | |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 12% PEG 20000, 100 mM MES (2-N-morpholino- ethanesulfonic acid) buffer, 3 mM HgCl2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
