1FP7
MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-21 |
| Detector | SIEMENS |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.876, 160.876, 256.118 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 3.200 |
| R-factor | 0.285 |
| Rwork | 0.285 |
| R-free | 0.35500 |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.116 | 0.376 |
| Total number of observations | 101298 * | |
| Number of reflections | 39423 | |
| Completeness [%] | 97.5 | 100 |
| Redundancy | 2100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 | 295 | AMMONIUM SULFATE, PEG 1000, POTASIUM MALEATE, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 46 (%sat) | |
| 2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 3 | 1 | reservoir | PEG1000 | 1 (%) | |
| 4 | 1 | reservoir | potassium maleate | 50 (mM) | pH7.6 |
