1FP6
THE NITROGENASE FE PROTEIN FROM AZOTOBACTER VINELANDII COMPLEXED WITH MGADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.250, 119.530, 120.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.26800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.390 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.064 | 0.279 |
| Total number of observations | 210392 * | |
| Number of reflections | 65315 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 88.8 | 69.4 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.5 | 298 | PEG 4000, 0.1M Tris HCl, Sodium Acetate, Glycerol, pH 8.5, LIQUID DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 30 (%) | |
| 2 | 1 | 1 | Tris-HCl | 0.1 (M) | |
| 3 | 1 | 1 | sodium acetate | 0.2 (M) | |
| 4 | 1 | 1 | glycerol | 18 (%) | |
| 5 | 1 | 1 | 10 (mM) |
