1FP5

CRYSTAL STRUCTURE ANALYSIS OF THE HUMAN IGE-FC CEPSILON3-CEPSILON4 FRAGMENT.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	113
Detector technology	CCD
Collection date	1999-08-07
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	105.600, 105.600, 47.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.242 *
Rwork	0.242
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	26.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.257
Total number of observations	106855 *
Number of reflections	12340 *
<I/σ(I)>	33.9	10.6
Completeness [%]	99.8	100
Redundancy	8.7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	22 *	PEG 4000, sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	IgE-Fc	10 (mg/ml)
2	1	drop	Tris	10 (mM)	pH8.0
3	1	reservoir	sodium acetate	25 (mM)	pH4.6
4	1	reservoir	PEG4000	33 (%)