1FOT
STRUCTURE OF THE UNLIGANDED CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 289 |
Detector technology | DIFFRACTOMETER |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 61.000, 61.000, 322.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.24300 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | MADNESS |
Data scaling software | FS |
Phasing software | PHASES |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.000 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.083 | |
Total number of observations | 86072 * | |
Number of reflections | 7854 | |
Completeness [%] | 82.1 | 52.7 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 16 * | Kuret, J., (1991) Biochemistry, 30, 10595. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | |
2 | 1 | drop | 2-propanol | 10 (%) | |
3 | 1 | drop | 10 (mM) |