1FOK
STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 1994-05-03 |
Detector | SOL GRUNER, FUJI |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.590, 119.340, 71.520 |
Unit cell angles | 90.00, 101.42, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.29600 |
Structure solution method | MIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 22.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.075 | 0.325 |
Number of reflections | 26253 | |
Completeness [%] | 98.5 | 96.1 |
Redundancy | 7.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.15 (mM) | |
10 | 1 | drop | glycerol | 5 (%) | |
11 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
12 | 1 | reservoir | MES | 1.0 (M) | |
2 | 1 | drop | DNA | 0.75 (mM) | |
3 | 1 | drop | ammonium sulfate | 1.1 (M) | |
4 | 1 | drop | MES | 0.5 (M) | |
5 | 1 | drop | 0.2 (M) | ||
6 | 1 | drop | potassium phosphate | 10 (mM) | |
7 | 1 | drop | dithiothreitol | 0.5 (mM) | |
8 | 1 | drop | EDTA | 0.5 (mM) | |
9 | 1 | drop | EGTA | 0.5 (mM) |