1FO6

CRYSTAL STRUCTURE ANALYSIS OF N-CARBAMoYL-D-AMINO-ACID AMIDOHYDROLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1998-08-12
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	70.230, 67.530, 137.480
Unit cell angles	90.00, 96.12, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.18668
R-free	0.23929
RMSD bond length	0.018
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.073	0.459
Number of reflections	93415
<I/σ(I)>	7.5
Completeness [%]	99.9	99.8 *
Redundancy	7.4	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	23 *	Lithium sulfate, HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.5K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	23 *	Lithium sulfate, HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.5K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	sodium azide	0.02 (%(w/v))
5	1	reservoir	lithium sulfate	1.15 (M)
6	1	reservoir	HEPES	100 (mM)