1FNP
CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT REACTION CENTER PRO L209-> PHE FROM THE PHOTOSYNTHETIC PURPLE BACTERIUM RHODOBACTER SPHAEROIDES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-07-08 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 141.748, 141.748, 187.415 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.24800 |
RMSD bond length | 0.009 |
RMSD bond angle | 23.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.050 | 0.272 |
Number of reflections | 64538 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 95.6 | 94.6 |
Redundancy | 1.6 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 18 * | Fritzsch, G.,(1998) Methods Enzymol., 297, 57. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | drop | 4 (M) | composed of 20mM NaH2PO4, 0.063%LDAO, 1.5M (NH4)2SO4, 3% 1,2,3-heptanetriol, and RC with an OD830=25. | |
3 | 1 | reservoir | 1.8-2.1 (M) |