1FNH
CRYSTAL STRUCTURE OF HEPARIN AND INTEGRIN BINDING SEGMENT OF HUMAN FIBRONECTIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | .9787, .9788, .9115, .9796 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.580, 86.290, 142.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 26.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 5479 | |
| <I/σ(I)> | 14 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 20 * | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-6 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (mM) | |
| 3 | 1 | reservoir | Na, K Tartrate | 0.8 (M) |
