1FNF
FRAGMENT OF HUMAN FIBRONECTIN ENCOMPASSING TYPE-III REPEATS 7 THROUGH 10
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10 |
| Detector | FUJI |
| Wavelength(s) | 0.9871, 0.9793, 0.9790, 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.050, 60.670, 58.440 |
| Unit cell angles | 90.00, 103.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.30800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.560 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.043 |
| Number of reflections | 28425 |
| Completeness [%] | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 5-10 (%) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | sodium cacodylate | 10 (mM) |
