1FN7
COUPLING OF DAMAGE RECOGNITION AND CATALYSIS BY A HUMAN BASE-EXCISION DNA REPAIR PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-07 |
Detector | SBC-1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.113, 92.113, 211.014 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.850 - 2.600 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.27000 |
RMSD bond length | 0.006 |
RMSD bond angle | 20.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.540 |
Rmerge | 0.073 | 0.352 |
Total number of observations | 83683 * | |
Number of reflections | 17653 | |
<I/σ(I)> | 14.3 | |
Completeness [%] | 96.6 | 99.8 |
Redundancy | 4.74 | 3.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 277 | 100 mM sodium cacodylate pH 6.5, 200 mM calcium acetate, 16-18% PEG, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium cacodylate | ||
2 | 1 | 1 | calcium acetate | ||
3 | 1 | 1 | PEG | ||
4 | 1 | 2 | PEG |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
5 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
6 | 1 | reservoir | calcium acetate | 200 (mM) | |
7 | 1 | reservoir | PEG8000 | 16-18 (%) |