1FMW
CRYSTAL STRUCTURE OF THE MGATP COMPLEX FOR THE MOTOR DOMAIN OF DICTYOSTELIUM MYOSIN II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.100, 180.400, 54.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.150 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.28600 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.460 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 2.150 | |
| Rmerge | 0.083 | |
| Total number of observations | 149338 * | |
| Number of reflections | 54467 | |
| Completeness [%] | 96.0 | 95 * |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | 277 | 8% Peg 8000, 50 mM Hepes, 1 mM DTT, pH 7.5, microbatch, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 8.0 (%(w/v)) | |
| 2 | 1 | 1 | HEPES | 50 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 1 | protein | 5.0 (mg/ml) |
