1FMB

EIAV PROTEASE COMPLEXED WITH THE INHIBITOR HBY-793

Experimental procedure

Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	1995-04-04
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	42.930, 45.710, 56.770
Unit cell angles	90.00, 110.50, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.136 *
Rwork	0.136
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HIV-1 PROTEASE
RMSD bond length	0.014
RMSD bond angle	0.046
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.067
Total number of observations	59519 *
Number of reflections	8443
Completeness [%]	88.5
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	EIAV PR	3.6 (mg/ml)
2	1	drop	urea	6 (M)
3	1	drop	potassium phosphate	20 (mM)
4	1	reservoir	PEG4000	20 (%)
5	1	reservoir	sodium acetate	25 (mM)
6	1	reservoir	ammonium sulfate	0.4 (M)
7	1	reservoir	sodium azide	0.1 (%)