1FLZ

URACIL DNA GLYCOSYLASE WITH UAAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1997-06-11
Detector	SIEMENS HI-STAR
Spacegroup name	P 43 21 2
Unit cell lengths	78.210, 78.210, 80.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.000 - 2.300
R-factor	0.22
RMSD bond length	0.006
RMSD bond angle	2.107
Data scaling software	X-GEN
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.140	0.260
Total number of observations	79612 *
Number of reflections	11146
<I/σ(I)>	14
Completeness [%]	96.0	92
Redundancy	7.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	0.1M Na HEPES, 2% polyethylene glycol 400, 2.0M ammonium sulfate, 0.002M UAAp, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)
2	1	reservoir	PEG400	2 (%)
3	1	reservoir	ammonium sulfate	2.0 (M)
4	1	drop	protein	14.9 (mg/ml)
5	1	drop	dUAAp	2.4 (mM)