1FLW

HEN EGG WHITE LYSOZYME MUTANT WITH ALANINE SUBSTITUTED FOR GLYCINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.080, 79.080, 38.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.812
R-factor	0.182 *
Rwork	0.165
R-free	0.23100
RMSD bond length	0.010
RMSD bond angle	1.508
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	1.812
Rmerge	0.052
Total number of observations	11609 *
Number of reflections	10852
Completeness [%]	93.5 *
Redundancy	1.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	295	sodium chloride, sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	glycine	0.05 (M)
2	1	drop	protein	0.7-1.5 (mg/ml)
3	1	reservoir	sodium acetate	50 (mM)
4	1	reservoir		0.6-0.9 (M)