1FLU
HEN EGG WHITE LYSOZYME MUTANT WITH ALANINE SUBSTITUTED FOR GLYCINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.310, 79.310, 38.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.785 |
Rwork | 0.174 |
R-free | 0.25000 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.523 |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 6.000 |
High resolution limit [Å] | 1.785 |
Rmerge | 0.056 |
Total number of observations | 12048 * |
Number of reflections | 10452 |
Completeness [%] | 86.8 * |
Redundancy | 1.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 295 | sodium chloride, sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | glycine | 0.05 (M) | |
2 | 1 | drop | protein | 0.7-1.5 (mg/ml) | |
3 | 1 | reservoir | sodium acetate | 50 (mM) | |
4 | 1 | reservoir | 0.6-0.9 (M) |