1FLT

VEGF IN COMPLEX WITH DOMAIN 2 OF THE FLT-1 RECEPTOR

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	160
Detector technology	CCD
Collection date	1997-02
Detector	ADSC QUANTUM
Spacegroup name	C 1 2 1
Unit cell lengths	81.440, 71.130, 77.860
Unit cell angles	90.00, 105.28, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.198
Rwork	0.198
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT/NCS AVERAGING
Starting model (for MR)	1vpf
RMSD bond length	0.013
RMSD bond angle	28.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.052 *	0.277 *
Total number of observations	332877 *
Number of reflections	47047	4520 *
<I/σ(I)>	8.3	3.5
Completeness [%]	99.0	95.8
Redundancy	7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	PEG4000	30 (%)
4	1	reservoir	ammonium sulfate	0.2 (M)
5	1	reservoir		0.02 (%)
6	1	reservoir	Tris-HCl	0.15 (M)