1FLM
DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.230, 84.600, 41.110 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.1503 |
| R-free | 0.18190 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.026 * | 0.163 |
| Total number of observations | 337547 * | |
| Number of reflections | 68514 | |
| <I/σ(I)> | 29 | 2 |
| Completeness [%] | 86.6 | 60.6 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 20% PEG6000, 0.1M TRIS(PH7.5), 20% GRYCEROL, 0.2M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | sodium chloride | 200 (mM) | |
| 4 | 1 | reservoir | MES | 0.1 (M) | |
| 5 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
| 6 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | glycerol | 0.2 (M) |
