1FL6
THE HAPTEN COMPLEXED GERMLINE PRECURSOR TO SULFIDE OXIDASE CATALYTIC ANTIBODY 28B4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-19 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.460, 127.610, 65.590 |
| Unit cell angles | 90.00, 104.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.216 |
| R-free | 0.27100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.390 |
| Total number of observations | 47152 * | |
| Number of reflections | 20056 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 93.6 | 91.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 298 | 100 mM Tris 800 mM NaCl 26% PEG 4000 4 mM hapten, pH 7.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 26 (%) | |
| 2 | 1 | 1 | Tris | 100 (mM) | pH7.5 |
| 3 | 1 | 1 | 800 (mM) | ||
| 4 | 1 | 1 | hapten | 4 (mM) |
