1FL0
CRYSTAL STRUCTURE OF THE EMAP2/RNA-BINDING DOMAIN OF THE P43 PROTEIN FROM HUMAN AMINOACYL-TRNA SYNTHETASE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.954, 59.683, 37.850 |
| Unit cell angles | 90.00, 114.83, 90.00 |
Refinement procedure
| Resolution | 34.350 - 1.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.22400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.160 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.062 * | 0.157 * |
| Total number of observations | 269210 * | |
| Number of reflections | 52816 | |
| <I/σ(I)> | 22.5 | 6.4 |
| Completeness [%] | 99.9 * | 99.1 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 35% PEG 3000 or 6000, 100mM TRIS pH7.5, 100-300 mM NaCl, 2mM DTE with a protein concentration of 11 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | PEG3000 | 28-35 (%) | or PEG6000 |
| 3 | 1 | reservoir | Tris | 100 (mM) | |
| 4 | 1 | reservoir | 100-300 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 2 (mM) |
