1FK5
STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH OLEIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-02 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.676, 49.717, 69.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.135 |
| Rwork | 0.135 |
| R-free | 0.18800 |
| Structure solution method | MR |
| Starting model (for MR) | (PDB CODE:1MZL) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.200 |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.300 |
| Rmerge | 0.057 |
| Total number of observations | 93324 * |
| Number of reflections | 18062 |
| Completeness [%] | 75.7 |
| Redundancy | 5.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 24.5-25.5 * | 3.6-4.8M Na formate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 3.6-4.8 (M) |
