1FK3

STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH PALMITOLEIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1999-08-09
Detector	ENRAF-NONIUS
Spacegroup name	P 21 21 21
Unit cell lengths	24.790, 49.790, 69.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.2 *
Rwork	0.200
R-free	0.23600
Structure solution method	MR
Starting model (for MR)	(PDB code:1mzl)
RMSD bond length	0.013
RMSD bond angle	1.600
Data reduction software	PROFILE-FITTING (PROCEDURE)
Data scaling software	PROFILE-FITTING (PROCEDURE)
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
High resolution limit [Å]	1.690
Rmerge	0.026
Total number of observations	22830 *
Number of reflections	9248
Completeness [%]	92.2
Redundancy	2.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		24.5-25.5 *	4.5M Na formate, 0.1M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	4.5 (M)
2	1	reservoir	lithium sulfate	0.1 (M)