1FK1

STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH LAURIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1999-09-05
Detector	ENRAF-NONIUS
Spacegroup name	P 21 21 21
Unit cell lengths	24.820, 49.680, 69.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.195 *
Rwork	0.182
R-free	0.21100 *
Structure solution method	MR
Starting model (for MR)	(PDB code:1MZL)
RMSD bond length	0.014
RMSD bond angle	1.630
Data reduction software	PROFILE-FITTING (PROCEDURE)
Data scaling software	PROFILE-FITTING (PROCEDURE)
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
High resolution limit [Å]	1.760
Rmerge	0.044
Total number of observations	23095 *
Number of reflections	8570 *
Completeness [%]	91.4
Redundancy	2.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		24.5-25.5 *	4.2M Na formate, 0.2M potassium cyanide, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	4.2 (M)
2	1	reservoir	potassium cyanide	0.2 (M)