1FJR

CRYSTAL STRUCTURE OF THE ECTODOMAIN OF METHUSELAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	123
Detector technology	CCD
Collection date	1999-10-19
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	68.937, 73.000, 115.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.212
R-free	0.22700
RMSD bond length	0.008
RMSD bond angle	26.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.069	0.338
Number of reflections	26305
<I/σ(I)>	15.5
Completeness [%]	99.5	98.6
Redundancy	5.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	22 *	drop consists of 4 micro litter of protein solution and 2 micro litter of precipitant *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir		1.6 (M)	precipitant
4	1	reservoir	HEPES	0.1 (M)	precipitant