1FJG
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-05 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 402.001, 402.001, 176.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.346 - 3.000 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.25500 |
Structure solution method | difference fourier |
RMSD bond length | 0.006 |
RMSD bond angle | 28.420 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.346 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.096 | 0.372 * |
Number of reflections | 259895 | |
<I/σ(I)> | 13.8 | |
Completeness [%] | 91.1 | 50.9 |
Redundancy | 4.2 | 1.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 277 | Wimberly, B.T., (2000) Nature, 407, 327. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NH4Cl | ||
10 | 1 | 2 | magnesium acetate | ||
11 | 1 | 2 | sodium cacodylate | ||
12 | 1 | 2 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | magnesium acetate | ||
5 | 1 | 1 | sodium cacodylate | ||
6 | 1 | 1 | MPD | ||
7 | 1 | 2 | NH4Cl | ||
8 | 1 | 2 | KCl | ||
9 | 1 | 2 | CaCl2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
4 | 1 | 2-mercaptoethanol | 6 (mM) | ||
5 | 1 | potassium cacodylate | 0.1 (M ) | or 0.1M MES |