1FJ9
FRUCTOSE-1,6-BISPHOSPHATASE (MUTANT Y57W) PRODUCTS/ZN/AMP COMPLEX (T-STATE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 180 |
Detector technology | AREA DETECTOR |
Collection date | 1998-04-20 |
Detector | SIEMENS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.820, 165.480, 79.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.500 |
Rwork | 0.194 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ieyj |
RMSD bond length | 0.006 |
RMSD bond angle | 23.000 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.700 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.069 | 0.180 |
Total number of observations | 88507 * | |
Number of reflections | 25328 * | |
<I/σ(I)> | 14.8 | 4 |
Completeness [%] | 90.0 | 83 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 37 * | PEG3350, MPD, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | HEPES | 100 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | F6P | 5 (mM) | |
5 | 1 | drop | 10 (mM) | ||
6 | 1 | drop | AMP | 5 (mM) | |
7 | 1 | reservoir | HEPES | 100 (mM) | |
8 | 1 | reservoir | PEG3350 | 6 (%(w/v)) |