1FJ0
STRUCTURE DETERMINATION OF THE FERRICYTOCHROME C2 FROM RHODOPSEUDOMONAS PALUSTRIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.310, 71.530, 66.660 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.178 * |
| Rwork | 0.178 |
| R-free | 0.21793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2c |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 0.025 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.087 | 0.358 |
| Total number of observations | 194662 * | |
| Number of reflections | 51959 | |
| <I/σ(I)> | 11 | 3.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 293 | ammonium sulfate, ferricyanide, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.0 (mg/ml) | pH6.0 |
| 2 | 1 | drop | phosphate | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 42-44 (%sat) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | pH4.4 |
