1FIX
THE STRUCTURE OF AN RNA/DNA HYBRID: A SUBSTRATE OF THE RIBONUCLEASE ACTIVITY OF HIV-1 REVERSE TRANSCRIPTASE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994-10-01 |
| Detector | SIEMENS |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 31.300, 31.300, 137.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.300 |
| R-factor | 0.139 * |
| Rwork | 0.145 |
| Structure solution method | MIR |
| Starting model (for MR) | A-FORM DUPLEX |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.200 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | XTAL |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 5.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.076 * |
| Total number of observations | 20393 * |
| Number of reflections | 3315 |
| Completeness [%] | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | pH 4.50, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | NA ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | methylpentanediol | 25.5 (%(w/v)) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | drop | sodium acetate | 0.1 (M) | |
| 4 | 1 | drop | duplex | 0.56 (mM) |
