1FIX
THE STRUCTURE OF AN RNA/DNA HYBRID: A SUBSTRATE OF THE RIBONUCLEASE ACTIVITY OF HIV-1 REVERSE TRANSCRIPTASE
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1994-10-01 |
Detector | SIEMENS |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 31.300, 31.300, 137.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.300 |
R-factor | 0.139 * |
Rwork | 0.145 |
Structure solution method | MIR |
Starting model (for MR) | A-FORM DUPLEX |
RMSD bond length | 0.019 |
RMSD bond angle | 2.200 |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | XTAL |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 5.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.076 * |
Total number of observations | 20393 * |
Number of reflections | 3315 |
Completeness [%] | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | pH 4.50, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | CACL2 | ||
4 | 1 | 1 | NA ACETATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | methylpentanediol | 25.5 (%(w/v)) | |
2 | 1 | drop | 0.2 (M) | ||
3 | 1 | drop | sodium acetate | 0.1 (M) | |
4 | 1 | drop | duplex | 0.56 (mM) |