1FIO
CRYSTAL STRUCTURE OF YEAST T-SNARE PROTEIN SSO1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-14 |
Detector | BRANDEIS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.517, 49.855, 113.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.26500 |
Structure solution method | multiwavelength anomalous diffraction |
RMSD bond length | 0.005 |
RMSD bond angle | 16.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.074 | 0.380 |
Number of reflections | 18088 | |
<I/σ(I)> | 17.9 | 4.6 |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | |
3 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
4 | 1 | reservoir | isopropanol | 10 (%(v/v)) |