1FIE

RECOMBINANT HUMAN COAGULATION FACTOR XIII

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-08-01
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	101.100, 73.130, 134.700
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.182
Rwork	0.182
RMSD bond length	0.011
RMSD bond angle	25.210 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.460 *	2.500 *
Rmerge	0.065
Total number of observations	101939 *
Number of reflections	50732
Completeness [%]	77.7	62 *
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6		pH 6.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethanol	10-15 (%)
2	1	reservoir	Tris-HCl	0.1 (M)