1FHE

GLUTATHIONE TRANSFERASE (FH47) FROM FASCIOLA HEPATICA

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-10-01
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	158.040, 158.040, 74.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.237
Rwork	0.237
R-free	0.35400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SCHISTOSOMA JAPONICUM GST
RMSD bond length	0.005
RMSD bond angle	28.560 *
Data reduction software	HKL
Data scaling software	HKL-2000
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.112 *	0.396 *
Number of reflections	9266
Completeness [%]	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.4	22 *	AMMONIUM SULFATE, GLUTATHIONE, TRIS PH 8.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-25 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	glutathione	10 (mM)